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. 2015 Jul 23;34(19):2424–2440. doi: 10.15252/embj.201490831

Figure 3.

Figure 3

Co-localization of FBW7 and NDE1 at the centrosome
  • A, B Co-localization of NDE1 and FBW7 at the centrosome of exponentially growing (asynchronous, A) or serum-starved (24 h, G1-arrested, B) RPE1-hTERT cells. (A) Cells were double stained with mouse α-FBW7 (red) and rabbit α-NDE1 (green) (a-d) or mouse α-FBW7 (green) and rabbit γ-tubulin (red) (e–g). Nuclei were stained with DAPI (blue). Scale bars: 10 μm (a) and 5 μm (b–g). (B) Presence of FBW7 in the basal body of serum-starved RPE1-hTERT cells visualized by double staining with cilia (ARL13B, a–c)- or centrosome [(γ-tubulin, d–f), (ninein, g–i), (centrin-2, j–l)]-specific markers. Rabbit or mouse primary antibodies are shown in green or red, respectively. Scale bar: 5 μm.
  • C Co-localization of NDE1 and FBW7 in the centrosome of DLD-1 cells. Wild-type or FBXW7−/− DLD-1 cells were double stained with rabbit α-NDE1 (green) and mouse α-FBW7 (red). Scale bar: 5 μm.
  • D Presence of FBW7 in the centrosome of mouse primary hippocampal neurons. Hippocampal neurons were identified by Tau-positive staining (a), and the expression of FBW7 in the centrosome and its co-localization with NDE1 were determined by double labeling with mouse α-FBW7 (red) and γ-tubulin (green) (b–d) or NDE1 (green) (e–g). Scale bar: 5 μm.