Table 2. The primer description of 10 candidate RGs evaluated in this study.

unigene number	gene symbol	gene description	sequence of forward (F) and reverse (R) primers	Amplicon length(bp)	Tm of primer	GC%
comp41339_c0	ABCS	Predicted transporter (ABC superfamily)	F 5'-CTTTGGCACGTGCTCTGTTC-3'	101	61.97	55
			R 5'-AAGGGTTTCTTCCAGCCACA-3'		61.94	50
comp35046_c0	60sRPL10	60s ribosomal protein L10	F 5'-GTTAGCATCGGTCAAGTCCTTC-3'	98	60.14	50
			R 5'-CCAGGGAACTTGAACTTAGCAC-3'		60.16	50
comp23736_c0	RanBP1	Ran-binding protein RANBP1 and related RanBD domain proteins	F 5'-TGCTTCCAGAACCTCCGATT-3'	140	62.05	50
			R 5'-GCTCCGGTGTCTTCGTCTTC-3'		62.3	60
comp36845_c0	UBQL	Ubiquitin-protein ligase	F 5'-ACCACCAGCTCCAATCACCA-3'	137	61.43	55
			R 5'-CGCTGCAAGAGGTAGGAGGA-3'		61.32	60
comp38667_c0	MFC	Multifunctional chaperone (14-3-3 family)	F 5'-ACATGGCCAAGCTCTCCGAG-3'	130	62.25	60
			R 5'-TAGGCGACGGAGAGGAGGTT-3'		62.21	60
comp31883_c1	UBCE2	Ubiquitin-conjugating enzyme E2	F 5'-GAGATGGCACCGTGAGTTATG-3'	102	60.53	52.38
			R 5'-GCCCTTCATGTACAGAGTTGTG-3'		59.66	50
comp40996_c0	eIF-5A	Translation initiation factor 5A (eIF-5A)	F 5'-TGTCCCTCATGTCAACCGTA-3'	120	59.96	50
			R 5'-GCATCATCAGTTGGGAGCTT-3'		60.23	50
comp33305_c0	COA	Acetyl-CoA acetyltransferase	F 5'-AACGGGGTTGCAAGTCCTGA-3'	118	61.35	55
			R 5'-CAAGTGGCGGTTGGAATGGG-3'		60.89	60
comp18801_c0	EF1β	Elongation factor 1 beta/delta chain	F 5'-TCTGGTGTCACTGCTGAAGG-3'	146	60.02	55
			R 5'-TCCTCACCGAAAAGATCCAC-3'		60.05	50
comp40939_c0	EF1	elongation factor 1 alpha	F 5'-GTGGTGGGCATCCATCTTGTT-3'	134	62.1	52.38
			R 5'-TACCTCCCAGGCTGATTGTG-3'		62.38	55