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. 2015 Oct 12;10(10):e0140218. doi: 10.1371/journal.pone.0140218

Table 3. The primer description of 5 candidate RGs evaluated in this study.

unigene number gene symbol gene description sequence of forward (F) and reverse (R) primers Amplicon length(bp) Tm of primer GC%
comp40978_c0 GAPDH Glyceraldehyde 3-phosphate dehydrogenase F 5'-CTGCCTTGCCCCTCTTGCTA-3' 141 62.2 60
R 5'-GCAGCTCTTCCACCTCTCCA-3' 61.55 60
comp31267_c0 ATPS F0F1-type ATP synthase, beta subunit F 5'-CCTGCTGACGATTTGACAGA-3' 147 59.98 50
R 5'-ATGGGGTGAGAGCATACGAG-3' 60.1 55
comp41245_c0 MBF1 Transcription factor MBF1 F 5'-CTATCATGCAGGGCCGTACT-3' 104 60.12 55
R 5'-AGCCTTCCCGGATTCATACT-3' 59.92 50
comp40111_c0 GTPB GTP-binding ADP-ribosylation factor Arf1 F 5'-GGGTCTCGATGCAGCTGGTA-3' 142 61.68 60
R 5'-ACCACCAACATCCCACACAGT-3' 61.88 52.38
comp31791_c0 GST Glutathione S-transferase F 5'-TTTCCGTGGCCCAGAGATCC-3' 98 61.91 60
R 5'-TCTGTGCTTCATCCGAGAGA-3' 58.16 50