Table 4. The primer information of CtFAD2 genes used in this study for validating the most stable RGs.
Primer name | Nucleotide sequence (5′-3′) | gene name | Accession no. |
---|---|---|---|
PF21-4 | TTCGTCCTCTACTACCTTGCC | CtFAD2-1 | HM165274 |
PR21-4 | CGCCGATGACTGTATTTCC | CtFAD2-1 | |
PF22-4 | TTCCACAACATCACCGACAC | CtFAD2-2 | HQ179940 |
PR22-4 | TCCTTCACCTCCTCATCTTTATC | CtFAD2-2 | |
PF23-4 | TGGAGTCTTTGGCACTTTGT | CtFAD2-3 | HQ179941 |
PR23-4 | CCGTGGAATCGTAGTGAGGG | CtFAD2-3 | |
PF24-4 | TCCAACACTTCACCACCAG | CtFAD2-4 | HQ831351 |
PR24-4 | GGAGCCAAACGACCATAT | CtFAD2-4 | |
PF25-4 | TTTGCTCCCTCCCGCCCTCT | CtFAD2-5 | HQ831352 |
PR25-4 | AGCCAACGGCATCGTCCACC | CtFAD2-5 | |
PF26-4 | GGGAGCAGGTGGTCGGATGT | CtFAD2-6) | HQ831353 |
PR26-4 | CGCCAGTGGAGTAGGAAGTTGAG | CtFAD2-6 | |
PF28-4 | AAGCCCAACAAACAAACCAT | CtFAD2-8 | HQ831355 |
PR28-4 | CACCCTTGAACGATCCAGTAA | CtFAD2-8 |