Table 5. Expression stability values of safflower RGs as calculated by NormFinder software.
Total | ABA | GA3 | PP333 | Salt | |||||
---|---|---|---|---|---|---|---|---|---|
Ranking | Stability value | Ranking | Stability value | Ranking | Stability value | Ranking | Stability value | Ranking | Stability value |
MFC | 0.431 | MFC | 3.392 | MFC | 1.251 | MBF1 | 0.769 | COA | 0.932 |
MBF1 | 0.381 | ABCS | 0.911 | MBF1 | 1.247 | COA | 0.742 | ABCS | 0.444 |
COA | 0.377 | EF1β | 0.827 | COA | 0.713 | GAPDH | 0.581 | GAPDH | 0.367 |
GAPDH | 0.374 | ATPS | 0.696 | GAPDH | 0.690 | MFC | 0.412 | UBQL | 0.299 |
GST | 0.210 | EF1 | 0.669 | RAN | 0.650 | UBQL | 0.339 | ATPS | 0.284 |
ABCS | 0.209 | UBQL | 0.666 | ABCS | 0.583 | GST | 0.307 | 60SRPL10 | 0.251 |
ATPS | 0.193 | COA | 0.652 | EF1β | 0.542 | RAN | 0.258 | UBCE2 | 0.238 |
UBCL | 0.177 | RAN | 0.649 | UBCE2 | 0.521 | ABCS | 0.248 | EF1β | 0.231 |
GTPB | 0.164 | EIF5A | 0.567 | UBQL | 0.504 | EF1β | 0.222 | GST | 0.210 |
RAN | 0.153 | GAPDH | 0.430 | EIF5A | 0.411 | 60SRPL10 | 0.163 | MFC | 0.206 |
EIFB | 0.150 | GTPB | 0.389 | EF1 | 0.286 | EIF5A | 0.163 | RAN | 0.195 |
60SRPL10 | 0.121 | MBF1 | 0.275 | GTPB | 0.150 | GTPB | 0.159 | EIF5A | 0.184 |
UBCE2 | 0.110 | GST | 0.260 | 60SRPL10 | 0.077 | EF1 | 0.129 | MBF1 | 0.161 |
EF1 | 0.081 | 60SRPL10 | 0.202 | GST | 0.031 | UBCE2 | 0.110 | GTPB | 0.132 |
EIF5A | 0.060 | UBCE2 | 0.180 | ATPS | 0.009 | ATPS | 0.031 | EF1 | 0.132 |
Best gene | Stability value | Best gene | Stability value | Best gene | Stability value | Best gene | Stability value | Best gene | Stability value |
EIF5A | 0.060 | UBCE2 | 0.180 | ATPS | 0.009 | ATPS | 0.031 | EF1 | 0.132 |
Best combination | Stability value | Best combination | Stability value | Best combination | Stability value | Best combination | Stability value | Best combination | Stability value |
EIF5A and EF1 | 0.050 | 60SRP10 and UBCE2 | 0.141 | ATPS and GST | 0.014 | ATPS and UBCE2 | 0.060 | EF1 and GTPB | 0.093 |