Table 6. Expression stability values of safflower RGs as calculated by NormFinder software.
| Cold | DDS | DFC | DT | ||||
|---|---|---|---|---|---|---|---|
| Ranking | Stability value | Ranking | Stability value | Ranking | Stability value | Ranking | Stability value |
| MBF1 | 0.729 | MBF1 | 2.147 | GAPDH | 1.264 | UBQL | 5.338 |
| GST | 0.433 | MFC | 1.617 | MFC | 0.883 | EIF5A | 3.655 |
| MFC | 0.378 | COA | 1.465 | COA | 0.515 | UBCE2 | 3.404 |
| ATPS | 0.372 | GST | 1.093 | ATPS | 0.461 | GTPB | 2.867 |
| GAPDH | 0.358 | RAN | 0.606 | GTPB | 0.434 | GST | 2.622 |
| COA | 0.350 | UBCE2 | 0.545 | GST | 0.381 | ABCS | 2.097 |
| ABCS | 0.332 | GAPDH | 0.481 | MBF1 | 0.363 | EF1 | 1.926 |
| GTPB | 0.263 | ABCS | 0.463 | ABCS | 0.268 | 60SRPL10 | 1.816 |
| RAN | 0.247 | UBQL | 0.321 | UBQL | 0.230 | GAPDH | 1.614 |
| UBCE2 | 0.242 | EIFB | 0.301 | EF1 | 0.221 | COA | 1.377 |
| UBCL | 0.233 | EF1 | 0.276 | EIF5A | 0.131 | MFC | 1.097 |
| EF1β | 0.201 | GTPB | 0.235 | 60SRPL10 | 0.071 | EIFB | 1.04 |
| 60SRPL10 | 0.144 | EIF5A | 0.179 | RAN | 0.054 | ATPS | 0.467 |
| EF1 | 0.142 | ATPS | 0.069 | UBCE2 | 0.025 | RAN | 0.398 |
| EIF5A | 0.079 | 60SRPL10 | 0.053 | EF1β | 0.025 | MBF1 | 0.398 |
| Best gene | Stability value | Best gene | Stability value | Best gene | Stability value | Best gene | Stability value |
| EIF5A | 0.079 | 60SRPL10 | 0.053 | UBCE2/EF1β | 0.025 | RAN/MBF1 | 0.398 |
Note: DDS: different development stage, DFC: different cultivars, and DT: different tissues.