Table II.
DNA concentration.
| Sample no. | Institute A (ng/µl) | Institute B (ng/µl) | Institute C (ng/µl) |
|---|---|---|---|
| 1 | 31 | 362.9 | 20.8 |
| 2 | 2.9 | 7.84 | 0.85 |
| 3 | 3.32 | 109.16 | 7.81 |
| 4 | 0.1 | 4.03 | 0.41 |
| 5 | 12.8 | 186.49 | 11.7 |
| 6 | 7.5 | 14.92 | 1.15 |
| 7 | 16.6 | 374.76 | 4.55 |
| 8 | 2.44 | 24.24 | 1.48 |
| 9 | 26.6 | 504.4 | 44.8 |
| 10 | 0.1 | 3.61 | <0.5 |
| 11 | 10.3 | 26.1 | 3.42 |
| 12 | 5.7 | 266.83 | 2.36 |
| 13 | 8.06 | 11.58 | 2.94 |
| 14 | 4.56 | 21.62 | 1.18 |
| 15 | 2.06 | 28.72 | 4.94 |
| 16 | 2.7 | 15.64 | 1.25 |
| 17 | 1.29 | 25.68 | 3.58 |
| 18 | 3.78 | 17.32 | 1.99 |
| 19 | 0.1 | 19.24 | 4.3 |
| 20 | 8.8 | 204.08 | 1.3 |
| 21 | 0.1 | 1.3 | 0.1 |
| 22 | 18.4 | 470.92 | 12.2 |
| 23 | 0.83 | 204.61 | 6.08 |
| 24 | 0.97 | 52.5 | 5.34 |
| 25 | 0.16 | 103.01 | 2.85 |
| 26 | 0.3 | 103.01 | 8.52 |
| 27 | 0.24 | 60.02 | 1.31 |
| 28 | 0.1 | 85.57 | 1.06 |
| 29 | 0.1 | 47.74 | 0.7 |
| 30 | 0.1 | 56.77 | 4.2 |
DNA extraction from 30 non-small cell lung cancer (NSCLC) samples was carried out with three different DNA extraction systems from Qiagen: BioRobot M48, QIA Symphony SP as well as manual extraction. After the extraction, concentration was measured with the Qubit 2.0 fluorometer in institutes A and C, or with the NanoDrop 2000c spectrophotometer in institute B.