Table IV.

EGFR mutation status.

Case	Expected result	A	B	C	Tumor cell content	A	B	C	A AF%	B AF%	C AF%
1	p.G719A	√	√	√	50	13936	4917	3001	20	15	24
1	p.V834L	√	√	√	50	9112	4917	4829	17	18	22
2	p.L838R	√	√	√	80	1430	10143	5885	17	17	17
3	p.E746_A750del	√	√	√	60	10584	3379	9216	79	45	44
4	p.E746_A750del	√	√	√	10	1102	512	5116	23	18	22
5	wt	√	√	√	90	wt	wt	wt
6	wt	√	√	√	70	wt	wt	wt
7	wt	√	√	√	60	wt	wt	wt
8	wt	√	√	√	30	wt	wt	wt
9	wt	√	√	√	30	wt	wt	wt
10	–	n.a.	n.a.	n.a.	80	n.a.	n.a.	n.a.
11	p.E746_A750del	√	√	√	60	9562	5020	1947	67	60	49
12	p.L858R	√ + p.T790M	√	√	50	29429/34779	8291	2820	28/1.03	21	12
13	p.E746_A750del	√	√	√	40	9936	11132	2820	31	29	25
14	p.L858R	√	√	√	30	35355	6911	5693	36	33	13
15	p.L858R	√	√	√	50	14143	1381	3407	31	41	31
16	p.E746_A750del	√	√	√	70	11546	1472	1975	34	51	33
17	p.L858R	n.a.	n.a.	√	70	n.a.	n.a.	3336	n.a.	n.a.	20
18	p.E746_A750del	√	√	√	n.d.	4179	406	1521	54	14	10
19	p.L747_A751delinsP	√	n.a.	√	70	7445	n.a.	1585	75	n.a.	54
20	p.L747_P753delinsS	√	√	√	80	8010	5816	4221	75	59	49
20	p.A755D	√	√	√	80	7297	5816	4221	74	59	59
21	p.E709A	√	√	√	80	716	3273	4662	23	24	22
21	p.G719S	√	√	√	80	2102	3273	4640	9	24	20
22	p.E746_A750del	√	√	√	50	8391	33615	1968	62	56	50
23	p.L858R	√ + p.T790M	√	√	30	20413/10246	11389	1994	27/1.42	20	18
24	p.L858R	√	√	√	30	9794	16509	1714	34	26	30
25	wt	n.a.	√	√	60	wt	wt	wt
26	wt	√	√	√	60	wt	wt	wt
27	wt	√	√	√	70	wt	wt	wt
28	wt	√	√	√	60	wt	wt	wt
29	wt	√	√	√	70	wt	wt	wt
30	wt	√	√	√	n.d.	wt	wt	wt

Concerning the epidermal growth factor receptor (EGFR) mutation status, we found concordance in 26/26 samples. The mutation status was analysed previously with conventional methods. Institute A found two resistance mutations in samples 12 and 23. AF%, allele frequency; hook, concordant EGFR result; n.a., not analysable, n.d., not determined; wt, wild-type.