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. 2015 Oct 12;10(10):e0140006. doi: 10.1371/journal.pone.0140006

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© 2015 Evans et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — Reagents and conditions: i) NH₄OAc, AcOH, 100°C, 16 hr; ii) N-Boc-3,6-dioxa-1,8-octanediamine, HBTU, DIPEA, DCM, rt, 5 hr; iii) 1:1 DMC/TFA, rt, 2 hr; iv) 5-FAM NHS ester, NEt₃, DMF, rt, 16 hr; v) Cy5 NHS ester, NEt₃, DMF, rt, 16 hr; vi) Biotin-derivative 7, HBTU, DIPEA, DMF, rt, 1 hr; vii) Succinic anhydride, DIPEA, MeCN, 30°C, 16 hr, then N-boc-3,6-dioxa-1,8-octanediamine, HBTU, DIPEA, DMF, 35°C, 16 hr; viii) 1:1 DCM/TFA, rt, 16 hr.