Fig 1. Aptamers are selected after 4 round of SELEX.

The stringency of the SELEX procedure was monitored using ELONA as described in “Materials and Methods” section. SELEX pool from round 4 (SELLiPABP) and the starting round (RND40) were assayed for binding to LiPABP protein. Recombinant LiPABP was plated at 500 ng/well (7.5 pmol/well) and incubated with 200 μL of digoxigenin labeled SELLiPABP aptamer population or digoxigenin-labeled RND40 library (0–80 nM). Finally, anti-digoxigenin-POD antibodies were added plate was revealed with ABTS solution at 405 nm. All the experiments were made in triplicate and average of two different experiments is shown.