Table 2.

Gene-specific primers designed for real-time PCR assay

Primer	Sequence
Reverse transcription
miR-214-3p-stem-loop	5ˊGTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACACTGC 3ˊ
miR 135a- stem-loop	5ˊGTCGTATGCAGAGCAGGGTCCGAGGTATTCGCACTGCATACGACTCACA 3ˊ
miR- 214	Forward : 5ˊGCACAGCAGGCACAGAC3ˊ
	Forward : 5ˊGCACAGCAGGCACAGAC3ˊ
miR-135a	Forward : 5ˊCGATATGGCTTTTTATTCCTA3ˊ
	Reverse : 5ˊGAGCAGGGTCCGAGGT 3ˊ
Insr	Forward : 5ˊAACAGATGCCACTAATCCTTC 3ˊ
	Reverse : 5ˊGCCCTTTGAGACAATAATCC 3ˊ
Irs2	Forward: 5ˊCAGCCAGGAGACAAGAACTC 3ˊ
	Reverse: 5ˊCGCTTCACTCTTTCACGAC 3ˊ
Akt2	Forward : 5ˊTTCGGCAAGGTCATTCTG 3ˊ
	Reverse: 5ˊTGAGGGCTGTAAGGAAGG 3ˊ

Akt2; RAC-beta serine/threonine-protein kinase, Insr; Insulin receptor, Irs2; Insulin receptor substrates and PCR; Polymerase chain reaction.