Table 1.
Primer sequences and characteristics of the polymerase chain reaction (PCR) reactions used for amplifications of myelin basic protein (Mbp), Olig2, Nestin and glial fibrillary acidic protein (Gfap) cDNAs
| Genes | Primer sequence | Product size (kb) | Number of cycles | T annealing (˚C) | cDNA (μl) |
|---|---|---|---|---|---|
| Mbp | F: CACAGAAGAGACCCTCACAG | 337/415 | 26 | 56 | 2 |
| R: CCCCAGCTAAATCTGCTGAG | |||||
| Olig2 | F: ACACCACCACGTGTCGGCTA | 450 | 30 | 56 | 3 |
| R: CTGCGTCTCTTCTAAGCCCAGA | |||||
| Nestin | F: GGAGTGTCGCTTAGAGGT | 483 | 30 | 58.4 | 2 |
| R: GGAGTGTCGCTTAGAGGT | |||||
| Gfap | F: CTCGTGTGGATCTGGAGAGGAA | 572 | 26 | 56 | 2 |
| R: GCCCTCCAGCAATTTCCTGTAG | |||||
| Gapdh | F: AAACCCATCACCATCTTCCA | 198 | 24 | 56 | 1 |
| R: GTGGTTCACACCCATCACAA | |||||