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. 2015 Oct 12;211(1):145–158. doi: 10.1083/jcb.201505104

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© 2015 O'Driscoll et al.

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Figure 7. — Immunogold detection of Hsp104 on NM-YFP assemblies in Hsp70 knockout cell sections. (A–D) Representative EM projection images of immunogold-labeled cell sections of Δssa1 (A and B) and Δssa2 (C and D) cells, as indicated. Detection was with an α-Hsp104 antibody. The red outlines, encompassing entire NM-YFP dot aggregates, were manually traced at the interface between the peripheral unstructured zone of the aggregates and the ribosomes in the cytosol.