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. 2015 Oct 12;211(1):7–18. doi: 10.1083/jcb.201503122

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© 2015 Jiang et al.

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Figure 3. — Ubr5 targets BuGZ and Bub3 for ubiquitination and turnover. (A) IP Ubr5 by BuGZ or Bub3 antibodies in HeLa cells. (B) The HECT domain of Ubr5 (Uf4) directly bound to BuGZ and Bub3. Asterisks indicate contaminating bands. (C) Ubr5 depletion stabilized Bub3 and BuGZ in tsBN2 cells. GFP-hUbr5, but not GFP-hUbr5ΔUf4, rescued the effect. GAPDH, loading control. (D) Ubr5 depletion reduced ubiquitinated GFP-Bub3 or GFP-BuGZ in HeLa cells. (E) Stronger interaction between Ubr5 and BuGZ/Bub3 in metaphase than in prometaphase. HeLa cells were collected as shown by red arrows. (F and G) BuGZ depletion led to elevated Bub3-Ubr5 association and Bub3 ubiquitination. Mitotic HeLa cells were used for IP. In G, Flag-Bub3–expressing cells were treated by MG132 before mitotic shake-off.