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. 2015 Oct 12;211(1):27–37. doi: 10.1083/jcb.201504136

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© 2015 Hyenne et al.

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Figure 4. — The exocyst regulates alae formation independently of RAL-1. (A) Quantitative electron microscopy analysis reveals that the MVB density is similar in sec-5(pk2358) and sec-8(ok2187) null mutants and WT controls. MVB density is increased after sec-8(RNAi) but not sec-15(RNAi) compared with control(RNAi) (P values, Mann-Whitney test). (B) Example of an MVB attached to the apical plasma membrane in sec-5(pk2358) (enlarged in B’). (C) Small vesicles (<100 nm diameter; black arrows) accumulating under the plasma membrane in sec-8(ok2187) mutants. (D and E) The exocyst subunits SEC-8 (D) and SEC-15 (E) do not colocalize with VHA-5 in the epidermis. The scale bars in D apply to E. APM, apical plasma membrane; Cu, cuticle; Cy, cytoplasm; MVBm, MVB outer membrane.