Table 1.
Sholl intersection number mean under least squares model
| Radius (µm) | Treatment | Sholl least squares mean | Standard error of the least squares mean | Comparison with the control | Comparison between ± NPD1 |
|---|---|---|---|---|---|
| 0–25 class interval 1 | Control | 3.83666667 | 0.07223875 | ||
| DMSO | 4.24888889 | 0.07223875 | 0.0263 | ||
| MPP+ | 2.61000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPP+ + NPD1 | 3.61272727 | 0.07223875 | 0.9678 | ||
| MPTP | 3.59000000 | 0.07223875 | 0.8977 | 0.0003 | |
| MPTP + NPD1 | 4.10909091 | 0.07223875 | 0.7397 | ||
| 26–50 class interval 2 | Control | 5.06333333 | 0.07223875 | ||
| DMSO | 4.35111111 | 0.07223875 | <0.0001 | ||
| MPP+ | 1.56000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPP+ + NPD1 | 4.02727273 | 0.07223875 | <0.0001 | ||
| MPTP | 2.26500000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPTP + NPD1 | 3.70181818 | 0.07223875 | <0.0001 | ||
| 51–75 class interval 3 | Control | 4.62847222 | 0.07372836 | ||
| DMSO | 4.10666667 | 0.07223875 | 0.0003 | ||
| MPP+ | 0.96000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPP+ + NPD1 | 3.25090909 | 0.07223875 | <0.0001 | ||
| MPTP | 1.04000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPTP + NPD1 | 2.71636364 | 0.07223875 | <0.0001 | ||
| 76–100 class interval 4 | Control | 3.28000000 | 0.07223875 | ||
| DMSO | 2.45333333 | 0.07223875 | <0.0001 | ||
| MPP+ | 0.59000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPP+ + NPD1 | 1.75090909 | 0.07223875 | <0.0001 | ||
| MPTP | 0.55000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPTP + NPD1 | 1.69818182 | 0.07223875 | <0.0001 | ||
| 100–125 class interval 5 | Control | 2.19333333 | 0.07223875 | ||
| DMSO | 0.92444444 | 0.07223875 | <0.0001 | ||
| MPP+ | 0.27000000 | 0.07223875 | <0.0001 | <0.0001 | |
| MPP+ + NPD1 | 0.94363636 | 0.07223875 | <0.0001 | ||
| MPTP | 0.37500000 | 0.07223875 | <0.0001 | 0.0129 | |
| MPTP + NPD1 | 0.80727273 | 0.07223875 | <0.0001 | ||
| 126–150 class interval 6 | Control | 0.73333333 | 0.07223875 | ||
| DMSO | 0.21333333 | 0.07223875 | 0.0003 | ||
| MPP+ | 0.12500000 | 0.07223875 | <0.0001 | 0.9284 | |
| MPP+ + NPD1 | 0.36363636 | 0.07223875 | <0.0001 | ||
| MPTP | 0.34500000 | 0.07223875 | <0.0001 | 1.000 | |
| MPTP + NPD1 | 0.44727273 | 0.07223875 | <0.0001 |
Mesencephalic neurons were treated with MPTP and MPP+ in the presence or absence of NPD1. The Sholl intersections numbers are shown for TH-positive neurons that survived the 24-h treatment. Data were broken down into 6 class intervals of 25-µm radius (left column) and the mean and standard error of the mean were fitted to the least squares regression model (columns 3, 4). The Scholl least squares mean values were compared pairwise against the control (column 5) and between samples in the presence or absence of NPD1 (column 6)