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. 2015 Oct 13;6:825. doi: 10.3389/fpls.2015.00825

FIGURE 2.

FIGURE 2

Callus induction from cotyledon explants and callus growth of Wt and ick1/2/5/6/7 plants. (A,B) Representative plates showing callus induction from cotyledon explants of Wt (left) and ick1/2/5/6/7 mutant (right) in the presence of 2,4-D and 6-BA (A) or 2,4-D alone (B). Similar results were obtained in three independent experiments. In each experiment, 10 plates were used for each treatment, with each plate having around 15 explants. The data from one experiment are shown here. (C) Callus induction frequency of the Wt and ick1/2/5/6/7 mutant explants on culture medium containing 2,4-D alone. The difference in the induction rate between the Wt and mutant at each time point was compared by Mann–Whitney U test. (D) Fresh weight of calli from the Wt and ick1/2/5/6/7 mutant cotyledon explants after 3–7 weeks of culture on culture medium containing both 2,4-D and 6-BA. The differences between the Wt and mutant were analyzed by Student’s t-test (error bar = SE; ∗P < 0.05, ∗∗P < 0.01).