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. 2015 Oct 13;6:825. doi: 10.3389/fpls.2015.00825

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Copyright © 2015 Cheng, Liu, Cao, Wang, Li, Zhang, Jiang, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Callus induction from cotyledon explants of the Wt and ick1/2/5/6/7 mutant at different 2,4-D concentrations. The cotyledons were excised form 7-day-old seedlings and cultured on 1/2 MS medium for 20 days. (A) Representative plates showing the cotyledon explants of Wt (the upper row) and ick1/2/5/6/7 mutant (the lower row) on 1/2 MS medium containing 0.005 mg/L, 0.02g/L, 0.04 mg/L (the first–third column) 2,4-D. (B) Callus induction rate of the cotyledon explants of ick1/2/5/6/7 and Wt on the indicated media. (C) Fresh weight of the cotyledon explants with callus of ick1/2/5/6/7 and Wt on the indicated media. The bars show the mean induction rates of 4–5 plates. Student’s t-test was used for analyzing the differences in induction rate and fresh weight between the Wt and mutant (error bar = SE; ^∗∗P < 0.01).