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. 2015 Oct 13;6:825. doi: 10.3389/fpls.2015.00825

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Copyright © 2015 Cheng, Liu, Cao, Wang, Li, Zhang, Jiang, Zhou and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Shoot regeneration from root-derived calli of Wt and various ick mutant plants. Root explants were cultured on callus induction medium (CIM) first. After 7 days, root explants with callus were transferred onto shoot induction medium (SIM) and cultured for 30 days. For each line, 9–10 plates were used with each plate having about 40 explants. (A) Representative plates showing shoot regeneration on SIM. The plates in the first row are: Wt (left), ick1 (middle), ick1/2 (right), and in the second row, ick1/2/7 (left), ick1/2/6/7 (middle), and ick1/2/5/6/7 (right). The red circles mark the calli with regenerating shoots. (B) Frequency of shoot regeneration in Wt and ick mutants (Mean ± SE). The significant differences among different lines were analyzed by Fisher’s least significant difference (LSD) method, and are indicated by different lowercase letters (P < 0.05).