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. 2015 Oct 13;6:851. doi: 10.3389/fpls.2015.00851

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Copyright © 2015 Huang, Tunc-Ozdemir, Chang and Jones.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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hxk1-3/rgs1-2 double mutants show intermediate growth phenotype between hxk1-3 and rgs1-2 sngle mutants. (A) Comparison of root elongation of Col-0, rgs1-2, hxk1-3, and hxk1-3/rgs1-2 mutant seedlings transferred to1/4 MS medium supplemented with 0, 0.25, 0.5, 0.75, 1, 2 or 3% D-glucose (w/v) under low intensity light (70 μmol s^-1 m^-2) in long-day chamber in 16/8 h (L/D). Values are means ± SD (n = 10–18). (B) Comparison of leaf size (the third leaf) of 2-week-old Col-0, rgs1-2, hxk1-3, and hxk1-3/rgs1-2 mutant seedlings grown under light (160 μmol s^-1 m^-2) in 16/8 h (L/D). Values are means ± SD (n = 10–12). ANOVA single factor analysis (α = 0.05) was conducted to compare the leaf size in hxk1-3and hxk1-3/rgs1-2. ^∗∗P < 0.01. (C) 3-week-old Col-0, hxk1-3, Ler, and gin2-1 plants grown under high intensity light (160 μmol s^-1 m^-2) in 16/8 h (L/D). Scale bar = 1 cm. (D) 2-week-old Col-0, hxk1-3, rgs1-2, and hxk1-3/rgs1-2 mutant plants grown under high intensity light (160 μmol s^-1 m^-2) in 16/8 h (L/D). Scale bar = 1 cm. (E) Green seedling assay of the Col-0, rgs1-2, hxk1-3, and hxk1-3/rgs1-2 mutant. The assay was performed as described in Section “Materials and Methods.” The average percentage of seedlings showing green cotyledons was determined and presented with means ± SD from one representative experiment of 4 biological replications. (F) Col, rgs1-2, hxk1-3, and rgs1-2/hxk1-3 have altered responses to saline stress. The assay was performed as described in Section “Material and Methods.” Values are means ± SD from quintuplicate.