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. 2015 Oct 13;6:851. doi: 10.3389/fpls.2015.00851

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Copyright © 2015 Huang, Tunc-Ozdemir, Chang and Jones.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cell cycle progress in rgs1-2 or hxk1-3 mutant showed changed response to D-glucose. (A) Meristematic activity in the primary root tip of Col-0, hxk1-3 and rgs1-2 plants as visualized by GUS-staining under the indicated sugar conditions as described in Section “Materials and Methods.” Seeds were germinated and cultured in 1/2 MS liquid medium for 3 days (3d); and then transferred to 1/2 MS liquid medium supplemented with 3% (w/v) D-glucose(+3d 3%G) or 3% (w/v) D-mannitol (+3d 3% M) for 3 more days. Scale bar = 0.1 mm. (B) The root meristem elongation as determined by the length-change of GUS-staining region in the primary root tip of Col-0, hxk1-3 and rgs1-2 seedlings. Values are means ± SD (n = 8–12) from one representative experiment of 3 biological replications. Pairwise Student’s t-test was used to compare values to the Col-0. ^∗P < 0.05; ^∗∗P < 0.01.