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. 2015 Oct 13;6:851. doi: 10.3389/fpls.2015.00851

FIGURE 4.

FIGURE 4

AtRGS1 internalizes in response to D-glucose even in the absence of AtHXK1. (A) Hypocotyls cells of 7-day-old Col-0 and hxk1-3 Arabidopsis seedlings stably expressing AtRGS1-YFP imaged after treatment with 3% D-glucose (w/v) for 30 min. Scale bar = 20 μm. (B) Quantization of the percentage of AtRGS1-YFP internalized in epidermal cells of Col-0 and hxk1-3 mutant seedlings hypocotyls before and after glucose stimulation. Values are percentage means ± SD, from a representative experiment (n = 10) of 3 biological replications. ANOVA single factor analysis (α = 0.05) was conducted to compare the difference between plant lines with the same glucose treatment.