FIGURE 6.

In vivo interaction between AtRGS1 and RHIP1, and AtHXK1 and RHIP1 determined by physical complementation of YFP using Bimolecular Fluorescence complementation (BiFC). The test pairs are indicated on the left. (Column 1) Differential interference contrast (DIC) images of transformed cells. (Column 2) BIFC (YFP). cYFP-tagged proteins were co-transformed with nYFP-tagged proteins into tobacco leaves as described in Section “Materials and Methods.” (Column 3) Mt-rk is an RFP mitochondria marker used for the transformation control. Fluorescence complementation of split YFP and expression of Mt-rk were observed by confocal fluorescence microscopy. (Column 4) DIC, BiFC, and Mt-rk are merged. Scale bars = 50 μm.