Table 1.
List of cultures prepared for this study, methane and volatile fatty acid (VFA) production, and details on OTU formation and alpha diversity.
| Digester | Condition | Methane (mL/g) (SD)a | Acetate (mM) (SD)b | Butyrate (mM) (SD)b | Propionate (mM) (SD)b | Domain | Readsc | OTUsd | Chao1d | ACEd |
|---|---|---|---|---|---|---|---|---|---|---|
| GZ | Cellulose | 481.5 (71) | 0 (0) | 0 (0) | 0 (0) | Archaea | 2,479 | 148 | 192 | 204 |
| Bacteria | 1,707 | 144 | 230 | 241 | ||||||
| Cellulose (55°C) | 315 (24) | 0.39 (0.01) | 3.2 (0.07) | 1.7 (0.42) | Archaea | 3,438 | 45 | 54 | 57 | |
| Bacteria | 2,365 | 29 | 39 | 47 | ||||||
| Food waste | 583.5 (6.4) | 0 (0) | 0 (0) | 0 (0) | Archaea | 3,672 | 132 | 200 | 192 | |
| Bacteria | 2,008 | 156 | 239 | 250 | ||||||
| Xylan | 296 (16) | 0 (0) | 0 (0) | 0 (0) | Archaea | 3,271 | 138 | 172 | 173 | |
| Bacteria | 1,858 | 123 | 190 | 202 | ||||||
| Xylose | 264 (47) | 0 (0) | 0 (0) | 0 (0) | Archaea | 3,025 | 130 | 168 | 170 | |
| Bacteria | 1,141 | 129 | 168 | 179 | ||||||
| SWH | Cellulose | 489.5 (77) | 1.3 (0.01) | 4.3 (0.52) | 0 (0) | Archaea | 3,045 | 74 | 110 | 113 |
| Bacteria | 3,506 | 59 | 94 | 106 | ||||||
| Cellulose (55°C) | 410 (28) | 0.86 (0) | 3.4 (0.2) | 0.82 (0.23) | Archaea | 2,318 | 34 | 36 | 36 | |
| Bacteria | 837 | 23 | 29 | 25 | ||||||
| Food waste | 576 (31) | 1.6 (0.13) | 7.9 (0.21) | 0.4 (0.14) | Archaea | 2,434 | 60 | 73 | 76 | |
| Bacteria | 2,995 | 63 | 102 | 105 | ||||||
| Xylan | 381 (25) | 0 (0) | 0 (0) | 0 (0) | Archaea | 2,070 | 145 | 194 | 204 | |
| Bacteria | 1,823 | 172 | 250 | 266 | ||||||
| Xylose | 276 (28) | 0 (0) | 0 (0) | 0 (0) | Archaea | 2,268 | 90 | 109 | 116 | |
| Bacteria | 4,164 | 50 | 89 | 91 |
aAverage of replicate measurements of total production. bAverage of replicate measurements of final accumulated concentration. cQuality-controlled reads contributing to the final OTU table. dAveraged over 10 rounds of random subsampling to the depth of the most depauperate sample within each domain.