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Plasmids used to construct the pathway for the production of (1R,3E,7E,11S,12S)-3,7,18-dolabellatriene in Escherichia coli BL21 (DE3).
| Gene(s) | Vector | Multiple cloning site | Restriction sites |
|---|---|---|---|
| dxr | pCola-Duet-1 | I | NcoI, EcoRI |
| dxs | pCola-Duet-1 | II | NdeI, XhoI |
| ispD/ispF operon | pCDF-Duet-1 | I | NcoI, EcoRI |
| idi | pCDF-Duet-1 | II | NdeI, XhoI |
| crtE | pET-Duet-1 | I | NcoI, EcoRI |
| cotB2 | pET-Duet-1 | II | NdeI, XhoI |
