FIGURE 2.

Chain length specificity of the hydrolase activity of PL-GOS. (A) pNP esters with different acid residue chain length where tested at 20°C in tris-HCl-Buffer, pH 7.3. Enzyme concentration was at 65 nM, substrate concentration was 200 μM. Activity is expressed relative to activity toward pNP-butyrate. Relative preference for shorter chain length did not change with temperature, as tested in tris-HCl-Buffer (B) or osmolarity of the buffer, as tested at 37°C (C). Experiments for (B) and (C) were conducted once.