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. 2015 Oct 13;2:31. doi: 10.3389/fnut.2015.00031

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Copyright © 2015 De Boer, Monk, Liddle, Power, Ma and Robinson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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mRNA expression of M1 markers Itgam and Nos2 (A), associated cytokines Tnfα, Il6, and Ccl2 (B), NLRP3 inflammasome genes Nlrp3, Il18, and Il1β (C), and key MHCII-related antigen presentation genes Cd74, Cd80, and Cd86 (D) from macrophages treated with ACM ± anti-Ad and Px in the chronic inflammation model. Macrophages were treated with ACM from LPS-challenged (10 ng/mL) CON or FO AT organ cultures for 24 h. 5 μg/mL of anti-Ad (or IgG) ± polymyxin B (36 μM; Px) were added to ACM treatments prior to culture with macrophages. Macrophages treated with media and 5 μg/mL IgG and 10 ng/mL LPS ± Px served as the control. Data show the means ± SEM (n = 5/diet/condition). Bars not sharing a letter are significantly different (p ≤ 0.05).