Table 1.
Description of NaCl-resistant plasmids (pSR1 to pSR8) and their observed sequence similarities.
| Plasmid (type of sample) | GenBank accession number no. | Size (bp) | G+C content (%) | ORFa | Length (aa)b | Closest similar protein (organism_accession number) | Domainc | E value | % Identity | No. of TM-helices |
|---|---|---|---|---|---|---|---|---|---|---|
| pSR1 (brine) | KM603475 | 2478 | 70.26 | 1* | 171 | Prolyl-tripeptidyl peptidase precursor (Candidatus accumulibacter); EXI70660; 322 aa | B | 3,00E-72 | 102/161 (63%) | 0 |
| 2* | 621 | DNA helicase II (Salinibacter ruber DSM 13855); YP_003572414; 1227 aa | B | 0.0 | 464/484 (96%) | 0 | ||||
| pSR2 (brine) | KM603476 | 1509 | 56.20 | 1* | 337 | Hypothetical protein (Natrinema pellirubrum); WP_006182474; 833 aa | A | 0.0 | 291/337 (86%) | 1 |
| 2 | 96 | Hypothetical protein (Halosimplex carlsbadense); WP_006886095; 168 aa | A | 5,00E-51 | 80/96 (83%) | 0 | ||||
| pSR3 (brine) | KM603477 | 1838 | 60.50 | 1* | 392 | Probable cell surface glycoprotein (Natronomonas moolapensis 8.8.11) YP_007488500 | A | 7,00E-108 | 212/382 (55%) | 2 |
| 2* | 97 | IISH7-type transposase (Natronomonas moolapensis 8.8.11) YP_007488498.1; 558 aa | A | 8,00E-50 | 85/97 (88%) | 0 | ||||
| pSR4 (rhizosphere) | KM603478 | 920 | 62.61 | 1 | 217 | Endonuclease III (Verrucomicrobia bacterium DG1235); WP_008102102; 229 aa | B | 1,00E-126 | 174/217 (80%) | 0 |
| pSR5 (rhizosphere) | KM603479 | 1800 | 64.15 | 1* | 304 | Hypothetical protein (Monosiga brevicollis MX1); XP_001749465; 1630 aa | E | 9,00E-27 | 96/270 (36%) | 1 |
| 2 | 164 | Site-specific recombinase (Pseudomonas fuscovaginae); WP_029533036; 197 aa | B | 4,00E-24 | 56/110 (51%) | 0 | ||||
| pSR6 (rhizosphere) | KM603480 | 2341 | 49.40 | 1* | 168 | OmpA/MotB domain-containing protein (Haliscomenobacter hydrossis DSM 1100) YP_004451090; 1170 aa | B | 5,00E-48 | 82/168 (49%) | 0 |
| 2 | 214 | Glycerol uptake facilitator or related permease (Methylacidiphilum infernorum V4); YP_001939268; 209 aa | B | 3,00E-38 | 86/202 (43%) | 6 | ||||
| 3* | 216 | Hypothetical protein (Paenibacillus daejeonensis) WP_020618935; 465 aa (putative sulfatase) | B | 1,00E-78 | 117/240 (49%) | 0 | ||||
| pSR7 (rhizosphere) | KM603481 | 1131 | 54.64 | 1* | 377 | RNA helicase (Methylophaga thiooxydans); WP_008290720; 605 aa | B | 0.0 | 323/383 (84%) | 0 |
| pSR8 (rhizosphere) | KM603482 | 426 | 70.66 | 1* | 142 | Pyrophosphate-energized proton pump (Ilumatobacter coccineus YM16–304) YP_007562997; 698 aa | B | 1,00E-50 | 95/142 (67%) | 3 |
aORFs involved in NaCl resistance are shown in boldface type, and asterisks indicate incomplete ORFs.
baa, amino acids.
cA: Archaea, B: Bacteria, and E: Eukarya.