FIGURE 5.

Western blot analysis of TACO protein in THP-1 cells. Cells were harvested after differentiation with PMA (30 nM/ml) for 72 h. Whole cell extracts were prepared and separated by SDS-PAGE on 10% gels, and Western blot assay was performed using TACO/Coronin-1A Antibody and goat anti-rabbit IgG-HRP antibody. Lane M: Marker; Lane1: SDS PAGE Profile; Lane 2: Western Blot showing the TACO protein of 57 kDa. Results are representative of at least three separate experiments that gave similar results.