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. 2015 Oct 13;5:15045. doi: 10.1038/srep15045

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(A) Immunoblots showing expression of phospho p38 and phospho p53 in UCB treated platelets. (B) Effect of UCB on cytosolic Bcl-2 family proteins expression (Bcl-2, tBid, phospho BAD and BAD) in platelets. (C) Expression of, Bax, cyt. C and phospho p53 in both mitochondria and cytosolic fractions of UCB treated platelets. COX-IV indicates cytochrome c oxidase subunit-IV used as mitochondrial loading control and β-tubulin as loading control for cytosolic fractions. Membrane was cut based on the molecular weight, probed with antibody of interest and band of interest with molecular weight is indicated with an arrow.