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. 2015 Oct 13;5:15045. doi: 10.1038/srep15045

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Copyright © 2015, Macmillan Publishers Limited

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Figure showing decreased circulating platelets and increased platelet apoptotic markers during phenylhydrazine (PHZ) induced hyperbilirubinemia. Effect of hyperbilirubinemia on (A) Platelet count and (B) Serum direct and indirect bilirubin level in control and PHZ treated rats. (C) Elevated apoptotic markers in washed platelets obtained from control and PHZ treated rats as assessed by fluorometric assays and presented as percentage increase/decrease in fluorescence (ROS, intracellular Ca²⁺, ΔΨm, cardiolipin peroxidation and PS externalization). (D) Expression levels of cytosolic apoptotic proteins in washed platelets of control and PHZ treated rats. Membrane was cut based on the molecular weight, probed with antibody of interest and band of interest with molecular weight is indicated with an arrow. **p < 0.01, ***p < 0.001; significant compared to saline control rats.