Figure 3. The correlation between total RNA inputs and threshold cycle (Ct) values of HEK293A cells as determined by S-Poly (T) and S-Poly (T) Plus methods.

Total RNAs were isolated from human HEK293A cells. The RNA inputs ranged from 2.5 ng (original) to 0.8 pg (diluted 5⁵ times) for each real-time PCR reaction. (A) miR-92a-3p, (B) miR-16-5p, (C) miR-27b-3p, (D) miR-210-3p, (E) miR-103a-3p, (F) miR-126-3p, (G) SNORD44 and (H) SNORD47.