Table 1. MRM validation of differentially expressed proteins associated with active immunization in R. chinensis.

Function	Protein name	Unigene ID	Peptide sequence	Fold change (Mean)	P-value
Stress response proteins	60S ribosomal protein L23	Znev_11393	DYDALDVANK	1.57	0.01
	isocitrate dehydrogenase	Znev_13297	VTIIPGDGIGPEISAAVQK	1.65	0.04
		Znev_13297	SLEGYETLYDNVDVVTIR	1.22	0.29
		Znev_13297	ENTEGEYSGIEHEIVDGVVQSIK	1.94	0.02
	glutathione S-transferase D1	Znev_15569	LYFDIGTLYQR	0.59	0.00
	cuticle protein 19	Znev_10404	AAPAVDYYAYPK	0.61	0.05
		Znev_10404	GEYSLVEPDGTVR	0.44	0.01
	protein-disulfide isomerase	Znev_03262	VLVSSNFDEVAFNK	1.34	0.14
Immune signaling molecules	GTPase Ras	Znev_14471	SFEDIGGYR	2.10	0.06
	ubiquitin conjugating enzyme	Znev_05594	TDQVIQALVALVNDPEPEHPLR	0.57	0.00
		Znev_05594	ADLAEEYLK	0.69	0.00
	26S proteasome	Znev_05722	IVAFVGSPVETEEK	0.94	0.45
Biosynthesis	hypothetical protein SINV_06138	Znev_09984	LFIGGLDYR	0.67	0.00
		Znev_09984	GFGFITYSR	0.92	0.50
		Znev_09984	GFGFVEFEDYDPVDK	0.54	0.01
	copa protein	Znev_06668	LVGQSIIAYLQQK	1.03	0.68
		Znev_06668	LSFLYLITGNLDK	0.52	0.01
Metabolism	transketolase-like protein 2	Znev_11074	SIPGSTVFYPSDAVSTER	3.46	0.00
	phosphoglycerate mutase 1	Znev_02731	FDIAHTSVLTR	1.21	0.61
Development	troponin i	Znev_15251	FDLEYAVK	0.61	0.00
		Znev_15251	DFEISDLNAQVNDLR	0.65	0.02
	PREDICTED: annexin-B9-like	Znev_05200	EFAGSLEDGYLSIVK	1.16	0.40