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. 2015 Oct 13;5:15029. doi: 10.1038/srep15029

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Copyright © 2015, Macmillan Publishers Limited

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(a) Immunoblot analysis of MTHFD2 in asynchronous, G0/G1, S or M phase arrested HeLa cells. β-Actin, ACTB, served as loading control. (b) Cropped immunoblot analysis of MTHFD2 in resting or activated T cells (anti-CD3/CD28 for indicated time periods in hours; or by PMA/ionomycin (P/I). Beta-Actin (ACTB) served as loading control. (c) mRNA levels of MTHFD2 in resting or activated T cells. (d) HCT-116, U-251 or HeLa cells in 5% serum or serum-starved in 0.2% serum for 48 hours and then treated with 20% serum for indicated time periods. Cells were then fixed, permeabilized and stained for MTHFD2, DNA (DAPI) and mitochondria (MitoT). One representative picture for each condition is shown.