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. 2015 Oct 13;5:14894. doi: 10.1038/srep14894

Figure 2. Knockout mutant construction strategy (A) and confirmation by PCR (B).

Figure 2

We constructed a plasmid, pNOalk, containing sequences flanking the ado and far genes for the ADO-type n-heptadecane biosynthesis pathway in Synechocystis 6803, around a kanamycin resistance cassette (A). We confirmed the absence of these genes from the mutant strain via PCR with 3 different primer sets (B) using genomic DNA from the wild type or the mutant strain (NOalk), or the plasmid pNOalk as templates. Binding sites of the three primer sets (1,2,3) on the wild-type chromosome are shown in panel (A).