Table 2. Oligonucleotides used in this study.
Primer name | Sequence |
---|---|
sll0208_US_forward | aacagctatgaccatgattacgaattCAAAATCTCCGGTCGGGTAAC |
sll0208_US_reverse | gaatatggctcatAGGGGCGTTGGACTCCTG |
MCS_sll0209_DS_forward | gctcggatccggtaccgtcgactctagaGCCGACAGGATAGGGCGTG |
sll0209_DS_reverse | tgtaaaacgacggccagtgccaagctGACAAAAGTGAATGGATGCCCG |
kanR_forward | gtccaacgcccctATGAGCCATATTCAACGGGAAAC |
kanR_MCS_reverse | tagagtcgacggtaccggatccgagctcTTAGAAAAACTCATCGAGCATCAAATG |
1.f | CGCCCAAGCGGTTGCTGAAGA |
1.r | GCGCCACAAACCGCTACCGT |
2.f | AGATGGCGGAACTCTTGCCGGA |
2.r | ATACCTTGGCGTCCCCCTGCA |
3.f | TCAGCTACGGCGAAGCCCTCA |
3.r | CCTAAAGAGCTACTAAAGGGC |
Sequences binding to template DNA are in capital letters, while sequences added to the 5′ end for assembly via SLIC (see methods) are in lowercase.