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. 2015 Oct 13;5:15217. doi: 10.1038/srep15217

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(A) Optimization of reaction time. (B) Optimization of reaction temperature. (C) Optimization of the concentration of TSPC. (D) Evaluation of the stability of TSPC labeled L-2HG. The isocratic elution method was used for the detection of L-2HG and TSPC labeled L-2HG.