Table 4.

Comparison of gene expression profiling between former and present studies in CD34⁺ cells of MPN subjects.

Studies	Guglielmelli et al. [17]	Jones et al. [18]	Steidl et al. [19]	Čokić et al.
MPN	PMF	PMF	PV	PV, ET, PMF
Purified from	PB-PMF BM-HC	PB-PMF BM-HC	BM-PMF BM-HC	PB-PV, ET, and PMF PB-HC
RNA	Pooled	Amplified 50 ng of total RNA	Individual	Amplified 300 ng of total RNA
Examined MPN patients	Mixed: de novo, treated/paused	Mixed: de novo, treated/paused	De novo	De novo
Number of patients	PMF, 3 × 5 HC, 3 × 5	PMF, 8 HC, 6	PV, 4 HC, 10	PV, 7, ET, 9, PMF, 4, and HC, 8
Microarray chips	Affymetrix HG-U133A GeneChip	Affymetrix HGU95Av2 chip	Atlas Clontech Human 1.2 I	Operon Human Genome 4.0
Number of examined genes	16.000 genes (22.238 probes)	9.670 genes (12.625 probes)	1.185 genes	25.100 genes (35.035 probes)
JAK2V617F	Mixture of 8 mutated and 7 nonmutated	Not analyzed	Not analyzed	Separated according to mutant allele burden
Characterized genes	174	95 (48 upregulated and 47 downregulated)	107	PV, 261, ET, 82, PMF, 94

HC: healthy controls, PB: peripheral blood, and BM: bone marrow.