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. Author manuscript; available in PMC: 2016 Mar 24.
Published in final edited form as: Nature. 2015 Sep 24;525(7570):523–527. doi: 10.1038/nature14978

Figure 1. CUL3KBTBD8 drives neural crest specification.

Figure 1

a. hESCs stably depleted of KBTBD8 were subjected to neural conversion and analyzed by qRT-PCR. (mean of 3 technical replicates, +/− s.e.m) b. Depletion of KBTBD8 results in loss of neural crest cells, as determined by Western analysis (full scans in Supplementary Fig. 1). c. KBTBD8-depleted hESCs were subjected to neural conversion and analyzed by immunofluorescence microscopy (mean of 3 biological replicates, +/− s.e.m; ~1500 cells/condition). d. X. tropicalis embryos injected with translation-blocking morpholinos against KBTBD8 were analyzed by in situ-hybridization. e. Model of the CUL3KBTBD8-controlled developmental switch.