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. Author manuscript; available in PMC: 2016 Mar 24.
Published in final edited form as: Nature. 2015 Sep 24;525(7570):523–527. doi: 10.1038/nature14978

Figure 4. Ubiquitylation-dependent TCOF1/NOLC1-complexes couple RNA polymerase I to ribosome modification enzymes.

Figure 4

a. Interactors of TCOF1 in 293T cells reconstituted with KBTBD8 or KBTBD8Y74A (sum of 3 biological replicates/condition). b. Validation of CUL3KBTBD8-dependent formation of TCOF1/NOLC1 complexes. c. CompPASS mass spectrometry analysis of sequential immunoprecipitation of FLAGTCOF1/HANOLC1-complexes. d. Validation of sequential affinity-purification of KBTBD8-dependent TCOF1/NOLC1-complexes (full scans in Supplementary Figure 1). e. Immunoprecipitation of RNA polymerase I from 293T cells reconstituted with KBTBD8 variants. f. Immunoprecipitation of RNA polymerase I from hESCs depleted of KBTBD8. g. Model of ubiquitin-dependent formation of a TCOF1/NOLC1-platform.