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. Author manuscript; available in PMC: 2016 Mar 24.
Published in final edited form as: Nature. 2015 Sep 24;525(7570):523–527. doi: 10.1038/nature14978

Figure 5. CUL3KBTBD8 remodels translational programs during differentiation.

Figure 5

a. 36S-methionine incorporation into newly synthesized proteins in hESCs lacking KBTBD8. (3 biological replicates +/− s.e.m.) b. RNA seq and ribosomal profiling of hESCs lacking KBTBD8 (blue: transcripts significantly changed in ribosome footprints but not mRNA; n>7725, q<0.1). c. Translation efficiency of ATRX1 and PCM1 in hESCs lacking KBTBD8. Orange: translation efficiency change; grey inserts: mRNA abundance change (mean of two biological replicates). d. Protein expression in hESCs lacking KBTBD8 during neural conversion (full scans in Supplementary Figure 1). e. Model of CUL3KBTBD8-dependent neural crest specification.