FIGURE 8.

Molecular pathological analyses for PEComas. Detection of the related gene fusion fragments was performed by RT-PCR. A distinct band of 186 bp in length (lane 2) was amplified in case 1 (A). DNA sequencing demonstrated that the transcript was composed of fusions of exon 9 of the PSF gene to exon 6 of the TFE3 gene (B and C). Semiquantitative RT-PCR was performed to analyze the expression levels of MiTF, TYR, C-MET, DTC, TBX2, CDK2, and Cyclin D1. MiTF, TYR, CDK2, TBX2, and C-MET were up-regulated in the tumor sample of case 1 (D). PEComa = perivascular epithelioid cell tumors; RT-PCR = reverse transcription-polymerase chain reaction.