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. 2015 Apr 24;94(16):e708. doi: 10.1097/MD.0000000000000708

FIGURE 3.

FIGURE 3

Effect of KCNJ5-M1 and KCNJ5-M2 on the aldosterone secretion and mRNA expression of CYP11B1 and CYP11B2. (A) Basal and A-II stimulated secretion of aldosterone production by H295R cells transfected with control, wild-type KCNJ5, KCNJ5-M1, and KCNJ5-M2 plasmids. Cells were incubated for 24 hours in the absence and presence of 100 nmol/L A-II after 24 hours of transfection. ∗∗P < 0.01, n = 5. (B) CYP11B1 and CYP11B2 mRNA expression levels in the H295R cells 24 hours after transfection with control, wild-type, and mutant KCNJ5 plasmids. Each bar represents the mean ± SE of relative gene expression in 3 independent experiments. The mRNA levels were normalized to glyceraldehyde-phosphate dehydrogenase (GAPDH) using the 2−ΔΔCT method. ∗∗P < 0.01.