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. 2015 May 22;94(20):e836. doi: 10.1097/MD.0000000000000836

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Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. http://creativecommons.org/licenses/by-nc-sa/4.0

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Schematic overview of results from discovery to validation. The number of common mutations was 6455 among 4 pooled samples from 237 sporadic PD patients. Validation and genotyping analysis of 4 novel nsSNPs and 6 annotated nsSNPs by Sanger sequencing. Genotyping was performed for 50 PD patients and 50 controls. Total numbers of validated and not validated nsSNPs are indicated. c.1981G>T in GRN and c.97C>T (rs11540014) in PM20D1 represent specific examples of not confirmed and confirmed calls, respectively.