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. 2014 Dec 2;93(28):e255. doi: 10.1097/MD.0000000000000255

FIGURE 6.

FIGURE 6

Influence of AKR1C2 silencing and CYP3A4 induction on MDA-MB-231 cell line proliferation after exposure to PCT in vitro. CYP3A4 expression was induced by transfection MDA-MB-231 cell line with the pcDNA3.1-CYP3A4 plasmid (A) and expression of AKR1C2 was decreased by the siRNA against AKR1C2 (B) as described in the “Methods.” Efficiency of cell manipulations was monitored by qPCR and immunoblotting (30 μg of protein per lane for CYP3A4 and 10 μg of protein per lane for AKR1C2). Transcript and protein levels of influenced cells with the respective controls are presented in the upper part. Cells were incubated without (PCT−) or with 100 nM PCT+ for 24 h and then cell proliferation was analyzed using flow cytometry (lower part). Two independent experiments were performed with consistent results. PCT = paclitaxel, siRNA = small interfering RNA.