Fig. 3. Comparison of confocal laser scanning microscope images of SkBr3 treated by MB-FITC-nanoliposome-Texas red-Her2_Ab complex (MLC-Her2_Ab) in groups.

Merge I images are combined images of DAPI, FITC, and Texas red mode. Merge II images are combined images of differential interference contrast (DIC) and Merge I mode. Merge III images are magnified images of Merge II. The group without Her2_Ab shows no fluorescence intensity. This is similar to the control group. The group without ultrasound (US) shows some fluorescence intensity, mainly in the region outside of SkBr3. Groups A-D showed some fluorescence intensity both outside and inside SkBr3 in a ratio depending on the US conditions of intensity, time, and duty cycle: group A, 1 w/cm², 1 minute, 20% duty cycle; group B, 1 w/cm², 1 minute, 60% duty cycle; group C, 1 w/cm², 2 minutes, 20% duty cycle; group D, 2 w/cm², 1 minutes, 20% duty cycle (DIC, DAPI, FITC, Texas red, Merge I, and Merge II, ×20; Merge III, ×63).