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Gels showing limit of detection of rotavirus VP6 gene fragment (379 bp) by conventional RT-PCR using RNA or total nucleic acid extracted from a stool sample containing a high concentration of rotavirus (EIA OD value = 3.0) diluted 10−1–10−7 in a negative stool suspension, by each extraction method. A = MagNA Pure Compact, B = KingFisher Flex, C = QIAamp Viral RNA, D= miniMAG®, E = easyMAG® and F = RNaid®. MM = 100 bp Plus DNA Ladder.
