Table 2.
Mean and standard deviations (SD) of Ct values obtained by quantitative reverse transcriptase PCR using undiluted and 1:10 dilutions of rotavirus RNA/TNA extracts from 3 stool samples.
| Sample | OD value | RNaid |
QIAamp Viral RNA |
MagNA Pure Compact |
KingFisher Flex 96 |
NucliSENS easyMAG |
NucliSENS miniMAG |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Original |
1:10 |
Original |
1:10 |
Original |
1:10 |
Original |
1:10 |
Original |
1:10 |
Original |
1:10 |
||
| Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | Mean ± SD | ||
| 5628 | 0.176 | 20.3 ± 0.124 | 24.23 ± 0.04 | 23.5 ± 0.02 | 21.0 ± 0.08 | 22.09 ± 0.79 | 24.88 ± 0.05 | 23.13 ± 0.05 | 26.52 ± 0.06 | 22.06 ± 0.04 | 24.84 ± 0.2 | 22.48 ± 0.07 | 25.13 ± 0.05 |
| 5627 | 0.854 | 17.21 ± 0.06 | 19.48 ± 0.04 | 16.0 ± 0.02 | 14.7 ± 0.03 | 16.3 ± 0.66 | 19.15 ± 0.06 | 16.97 ± 0.07 | 20.62 ± 0.03 | 16.93 ± 0.06 | 19.08 ± 0.03 | 17.24 ± 0.03 | 19.61 ± 0.04 |
| 5630 | 3.098 | 15.38 ± 0.04 | 18.35 ± 0.02 | 17.7 ± 0.04 | 15.5 ± 0.06 | 15.3 ± 0.48 | 17.53 ± 0.06 | 15.19 ± 0.02 | 17.73 ± 0.1 | 14.64 ± 0.05 | 16.17 ± 0.07 | 15.21 ± 0.03 | 17.14 ± 0.04 |