Table 3.

Mean and standard deviation (SD) of C_T values obtained by qRT-PCR using TNA or RNA extracted from a stool sample with a high viral concentration diluted 10⁻¹ to 10⁻⁷ in a negative stool sample by each extraction method.

Dilution	OD value	RNaida	MagNA Pure Compactb	NucliSENS miniMAG	NucliSENS easyMAG	KingFisher Flexc	QIAamp Viral RNA
		Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD	Mean ± SD
10−1	2.81	13 ± 0.09	13.4 ± 0.23	17.3 ± 0.01	15.3 ± 0.18	12 ± 0.03	12.3 ± 0.11
10−2	1.51	17 ± 0.03	17.4 ± 0.34	19.5 ± 0.08	18.8 ± 0.01	17.3 ± 0.13	18 ± 0.04
10−3	0.32	20 ± 0.20	20.5 ± 0.07	22.3 ± 0.04	21.4 ± 0.22	20.7 ± 0.96	21.2 ± 0.13
10−4	0.10	23.1 ± 0.11	23.3 ± 0.06	25.8 ± 0.08	24.5 ± 0.23	23.6 ± 0.29	24 ± 0.01
10−5	0.08	24.9 ± 0.02	27 ± 0.21	27.4 ± 0.07	28.1 ± 0.22	25.6 ± 0.17	28.2 ± 0.09
10−6	0.08	28.4 ± 0.17	28.6 ± 0.07	31.6 ± 0.12	31.4 ± 0.12	30 ± 0.08	31.6 ± 0.51
10−7	0.07	29 ± 0.01	33.7 ± 0.19	33.2 ± 0.11	34.6 ± 0.40	33.3 ± 0.24	33.2 ± 0.24

^aC_T values obtained for RNaid kit extracts were significantly lower than those obtained for the easyMAG (P < 0.001), miniMAG (P < 0.001), and QIAamp (P < 0.01) kits.

^bC_T values obtained for MagNA Pure Compact extracts were significantly lower than those obtained for the miniMAG (P < 0.05).

^cC_T values obtained for KingFisher Flex extracts were significantly lower than those obtained for the easyMAG (P < 0.05) and miniMAG (P < 0.01).