Table 1.

Colony forming potential in TNBCs and MCF10A control cells

Treatments (10 days)	Cells plated	Colony counted	Plating efficiency (%)	Survival fraction (%)
M DA-MB-15 7
DMSO	500	490	98	100.00
Res 15	500	399	79.8	81.43
Ptero 5	500	417	83.4	85.10
Combination	500	220	44	44.90
Treatments	Cells plated	Colony	Plating	Survival fraction
(14 days)		counted	efficiency
MCF10A
DMSO	500	165	32.96	100
Res 15	450	136	30.33	92.03
Ptero 5	500	175	35.06	106.3
Combination	480	145	30.13	91.44

After staining the tissue culture dishes (20 mm), colonies were counted using a colony counter. Large colonies consisting of 50 or more cells were counted. MDA-MB-157 breast cancer cells displayed a reduction in colony forming potential after different treatments as determined by survival fraction (%). This reduction was more effective in combination treatments when compared with single doses of resveratrol (15 μM) and pterostilbene (5 μM) alone. Interestingly, MCF10A control cells did not display a significant change in the colony forming potential when compared within the treatment groups and the DMSO treated group. These observations further provide the effectiveness of this combinatorial regimen in inhibiting breast cancer cells growth. However, HCC1806 breast cancer cells did not form any successful colonies in any of the groups (data not shown). Values are representative of three independent experiments